Extraction Methods for Recovering Malachite Green and Leucomalachite Green

Reduction of malachite green to leucomalachite green by intestinal bacteria.

Intestinal microfloras from human, rat, mouse, and monkey fecal samples and 14 pure cultures of anaerobic bacteria representative of those found in the human gastrointestinal tract metabolized the triphenylmethane dye malachite green to leucomalachite green. The reduction of malachite green to the leuco derivative suggests that intestinal microflora could play an important role in the metabolic...

Determination of malachite green and leucomalachite green residues in rainbow trout in Sanandaj City

Background and Aim: Malachite green (MG) is an organic compound which his used as a dye in textile industry. This compound is applied incorrectly and illegally in aquiculture as an antimicrobial agent. This compound has mutagenic and carcinogenic effects. Materials and Methods: In this study we determined the concentrations of MG and leucomalachite green (LMG) in the muscle tissue of 90 rainbow...

Enhanced Sensitive Immunoassay: Noncompetitive Phage Anti-Immune Complex Assay for the Determination of Malachite Green and Leucomalachite Green

To develop a more sensitive immunoassay for malachite green (MG) and leucomalachite green (LMG), we identified the immunocomplex binding phage-borne peptides for use in the noncompetitive phage anti-immunocomplex assay (PHAIA). An anti-LMG monoclonal antibody (mAb) was used to select immunocomplex binding peptides from a circular random eight-amino-acid phage-displayed library. After three roun...

Effects of Malachite Green and Its Major Metabolite, Leucomalachite Green, in Micromass Cultures of Rat Embryonic Cells

Embryonic mesenchyme limb (LB) and midbrain (CNS) cells cultured at high density were exposed to different concentrations of the fungicide malachite green (MG) and its major metabolite leucomalachite green (LMG). The concentration response curves were analysed, and two endpoints were calculated: the concentrations (μg/mL) that produced 50% inhibition of viability/proliferation (IC50-P) and diff...

Risk Assessment of Malachite Green in Food